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In North America, an unusual pattern was observed in an individual from Florida (USA) that carried haplotype H24, which is shared with animals from southern Central America (Panama and Costa Rica) and is genetically distant from the other lineages found throughout North America. Given the record of human-induced introgression of puma mtDNA lineages from Central America to puma basket heart Florida in the 1960s,it is possible that this individual descends from a female translocated during that period.

Both hypotheses remain viable and should be the focus of additional scrutiny. Moreover, northwestern South America has been reported to harbor high levels of genetic diversity and a complex evolutionary history for several mammalian taxa ( e.g. Eizirik et al. , 1998 ; Ruiz-García et al. , 2009 , 2010 , 2012 ; Thoisy et al. , 2010 ), highlighting the need to further investigate pumas from this portion of their range to assess their role in the overall phylogeographic structure of the species.